The clonal human embryonic progenitor cell line 7SMOO7 was derived from the registered parental hES cell line H9 (WA09) as described (West et al, 2008). It expresses numerous cancer/testis genes reported to be expressed only in germ-line cells and cancer (Andrade et al, 2008; Jacobs et al, 2008), including the melanoma antigen family genes: MAGEA1, A10, MAGEC1, C2, and the prostate antigen family genes: PAGE2, 2B, 5. Upon differentiation with kit ES-K23, the cells express relatively high levels of smooth muscle markers including MYH11, ACTC1, DRP2, and MYLC2PL as well as the genes: PPEF1, and TAC1.
Positive mRNA Markers: MAGEA1, MAGEA10, MAGEC1, MAGEC2, PAGE2, PAGE2B, PGE5
Product Information:
Derivation: Progenitor line W10 was derived from the parental NIH registered human ES cell line H9 (WA09) as previously described (West, M.D. et al, 2008).
Cell Number: Vial contains >500,000 cells cryopreserved in 1 ml of FBS with 10% DMSO.
Recommended Growth Medium: ES-NC140
Recommended Culture Conditions: Cells should be plated onto tissue culture grade polystyrene plastic coated with 0.1% gelatin. Following rapid thawing and slow dilution in the final culture medium, the initial seeding density should be approximately 20,000 cells/cm2. Cells should be maintained at 37°C in a humidified incubator preferably with 5% CO2 and 5% oxygen with media change at least twice per week. Upon reaching confluence they should be split 1:3 for routine maintenance. Note: confluence for more than two days may lead to terminal differentiation.
Available Differentiation Kits:
Differentiation Kits: Cat No. ES-K23
Population Doubling Time: Approximately 50 hours.
Population Doubling: Progenitor line 7SMOO7 is sold at passage 12 (original clonal isolate in confluent 1.9 cm2 well being P1), which corresponds to approximately 15 doublings since the first 1.9 cm2 well and approximately 31.5 doublings since its original clonal plating. The line displays a finite lifespan in vitro, senescing at approximately 70 PD. When used properly this product should scale for a minimum of 10 population doublings.
Quality Control:
Sterility: The line is negative for bacteria and fungal contamination.
Thaw Test Result: >50% viability, >25% attachment, growth to confluence, and maintenance of
original morphologic appearance.
Certificates of Analysis: Available on request.
Restrictions: These cells are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.
Technical Assistance: Please contact a representative at ES Cell International (technicalsupport@embryome.com or (510) 521-3390) for technical assistance.
Pricing:
Cat Number Description List Price
ES-298 7SMOO7 Embryonic Progenitor Cell Line $1,350.00
ES-NC140 ESpan Cell Culture Medium Kit (with supplements) 129.00
ES-K23 Differentiation Kit 295.00
References:
Andrade VC, Vettore AL, Felix RS, Almeida MS, Carvalho F, Oliveira JS, Chauffaille ML, Andriolo A, Caballero OL, Zago MA, Colleoni GW. 2008. Prognostic impact of cancer/testis antigen expression in advanced stage multiple myeloma patients. Cancer Immun. 8:2.
Jacobs JF, Grauer OM, Brasseur F, Hoogerbrugge PM, Wesseling P, Gidding CE, van de Rakt MW, Figdor CG, Coulie PG, de Vries IJ, Adema GJ. 2008. Selective cancer-germline gene expression in pediatric brain tumors. J Neurooncol. 88(3):273-80.
West, M.D., Sargent, R.G., Long, J., Brown, C., Chu, J-S., Kessler, S., Derugin, N., Sampathkumar, J., Burrows, C., Vaziri, H., Williams, R., Chapman, K.B., Larocca, D., Loring, J.F., and Murai, J. 2008. The ACTCellerate Initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Reg. Med. 3(3): 287-308.
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