The clonal human embryonic progenitor cell line SM30 was derived from the registered parental hES cell line H9 (WA09) as described (West et al, 2008). It expresses FGF18, ITGB4, and no HOX gene expression. Upon differentiation with kit ES-K43, the line is intensely chondrogenic expressing high levels of cartilage markers including: COL2A1, LECT1, MATN4, EPYC, COL9A1, and Chondroadherin (CHAD). The line lacks the mesenchymal stem cell marker CD74 and unlike the line E15, is NNAT negative.

Positive mRNA Markers: SOX9, FGF18, NDST3, ITGB4

Product Information:

Derivation: Progenitor line W10 was derived from the parental NIH registered human ES cell line H9 (WA09) as previously described (West, M.D. et al, 2008).

Cell Number: Vial contains >500,000 cells cryopreserved in 1 ml of FBS with 10% DMSO.

Recommended Growth Medium: ES-M32

Recommended Culture Conditions: Cells should be plated onto tissue culture grade polystyrene plastic coated with 0.1% gelatin. Following rapid thawing and slow dilution in the final culture medium, the initial seeding density should be approximately 20,000 cells/cm2. Cells should be maintained at 37°C in a humidified incubator preferably with 5% CO₂ and 5% oxygen with media change at least twice per week. Upon reaching confluence they should be split 1:3 for routine maintenance. Note: confluence for more than two days may lead to terminal differentiation.

Available Differentiation Kits:

Chondrogenesis kit: Cat No. ES-K43

Population Doubling Time: Approximately 50 hours.

Population Doubling: Progenitor line SM30 is sold at passage 11 (original clonal isolate in confluent 1.9 cm2 well being P1), which corresponds to approximately 13.5 doublings since the first 1.9 cm2 well and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, senescing at approximately 70 PD. When used properly this product should scale for a minimum of 10 population doublings.

Quality Control:

Sterility: The line is negative for HIV (1, 2), HBV, HCV, EBV, bacteria, mycoplasma, and fungal contamination.

Thaw Test Result: >50% viability, >25% attachment, growth to confluence, and maintenance of original morpho­logic appearance.

Certificates of Analysis: Available on request.

Restrictions: These cells are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.

Technical Assistance: Please contact a representative at ES Cell International (technicalsupport@embryome.com or (510) 521-3390) for technical assistance.

Pricing:

Cat Number Description List Price

ES-256 SM30 Embryonic Progenitor Cell Line $1,350.00

ES-M32 ESpan Cell Culture Medium Kit (with supplements) 129.00

ES-K43 Differentiation Kit 295.00

References:

West, M.D., Sargent, R.G., Long, J., Brown, C., Chu, J-S., Kessler, S., Derugin, N., Sampathkumar, J., Burrows, C., Vaziri, H., Williams, R., Chapman, K.B., Larocca, D., Loring, J.F., and Murai, J. 2008. The ACTCellerate Initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Reg. Med. 3(3): 287-308.