The clonal human embryonic progenitor cell line SK11 was derived from the registered parental hES cell line H9 (WA09) as described (West et al, 2008). It expresses Brachyury (TBX15) and no HOX gene expression. Upon differentiation with kit ES-K41 the line upregulates the monocarboxylate transporter SLC16A9, useful in drug screening. Upon differentiation with kit ES-K75, the cells express high levels of PDE8B useful for psychotropic drugs (Fatemi et al, 2010).
Positive mRNA Markers: TBX15, PI16, LGSN
Product Information:
Derivation: Progenitor line SK11 was derived from the parental NIH registered human ES cell line H9 (WA09) as previously described (West, M.D. et al, 2008).
Cell Number: Vial contains >500,000 cells cryopreserved in 1 ml of FBS with 10% DMSO.
Recommended Growth Medium: ES-M60
Recommended Culture Conditions: Cells should be plated onto tissue culture grade polystyrene plastic coated with 0.1% gelatin. Following rapid thawing and slow dilution in the final culture medium, the initial seeding density should be approximately 20,000 cells/cm2. Cells should be maintained at 37°C in a humidified incubator preferably with 5% CO2 and 5% oxygen with media change at least twice per week. Upon reaching confluence they should be split 1:3 for routine maintenance. Note: confluence for more than two days may lead to terminal differentiation.
Available Differentiation Kits:
Differentiation Kits: Cat No. ES-K41, K75
Population Doubling Time: Approximately 50 hours.
Population Doubling: Progenitor line SK11 is sold at passage 11 (original clonal isolate in confluent 1.9 cm2 well being P1), which corresponds to approximately 13.5 doublings since the first 1.9 cm2 well and approximately 30 doublings since its original clonal plating. The line displays a finite lifespan in vitro, senescing at approximately 70 PD. When used properly this product should scale for a minimum of 10 population doublings.
Quality Control:
Sterility: The line is negative for bacteria and fungal contamination.
Thaw Test Result: >50% viability, >25% attachment, growth to confluence, and maintenance of original morphologic appearance.
Certificates of Analysis: Available on request.
Restrictions: These cells are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.
Technical Assistance: Please contact a representative at ES Cell International (technicalsupport@embryome.com or (510) 521-3390) for technical assistance.
Pricing:
Cat Number Description List Price
ES-250 SK11 Embryonic Progenitor Cell Line $1,350.00
ES-M60 ESpan Cell Culture Medium Kit (with supplements) 129.00
ES-K41 Differentiation Kit 295.00
References:
Fatemi SH, Folsom TD, Reutiman TJ, Braun NN, Lavergne LG. 2010. Levels of phosphodiesterase 4A and 4B are altered by chronic treatment with psychotropic medications in rat frontal cortex. Synapse. 2010 Jul;64(7):550-5.
West, M.D., Sargent, R.G., Long, J., Brown, C., Chu, J-S., Kessler, S., Derugin, N., Sampathkumar, J., Burrows, C., Vaziri, H., Williams, R., Chapman, K.B., Larocca, D., Loring, J.F., and Murai, J. 2008. The ACTCellerate Initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Reg. Med. 3(3): 287-308.
|
